The purpose of the study was to determine genetic struc- ture in terms of gliadin loci of spring wheat varieties of Siberi- an selection belonging to three groups of grain quality-strong, valuable and fillers. The problems were identifying alleles of gliadin loci by electrophoretic spectra; the analysis of the frequency of their occurrence in loci, in certain varieties and quality groups. The material included 23 varieties of wheat, created in 8 regions of Siberia. The electrophoresis of gliadins was carried out in polyacrylamide gel in aluminum-lactate buffer pH 3.1. For the identification of alleles, the reference variety Bezostaya 1 was used. It was established that all in- vestigated varieties had unique spectra, and, in addition to three, they were homogeneous in gliadins. Each locus was represented by 2-9 alleles, to analyze the relationship withgrain quality; alleles present in more than 9-20 genotypes were used: Gli-A1f, Gli-B1e, Gli-B1b, Gli-D1a, Gli-A2q, Gli- D2a. Two of them were present in 29 genotypes (Gli-B1e-9 and Gli-B1b-20), which indicated the importance of Gli-B1 locus for Siberian varieties. If Gli-B1b allele was practically uniformly found in three groups, the second allele of this Gli- B1e locus was more often observed in strong varieties (56 %) compared to valuable (22 %) and fillers (22 %). Gli-D1a allele is characteristic for valuable varieties (47 %), compared to strong (33 %) and fillers (20 %). Gli-D1a allele is characteris- tic for valuable varieties (47 %). The alleles of two Gli-A2q and Gli-D2a loci had the same frequency of occurrence in general sample and in quality groups, in fillers more often (54 %) than in the others. Gli-A1f allele was more common in strong varieties (44 %) compared with valuable varieties (33 %) and fillers (22 %). Thus, Gli-B1e allele was distin- guished according to the frequency in strong varieties, Gli- D1a allele was selected, in valuable - Gli-D1a alleles, Gli-A2q and Gli-D2a in fillers.