The assessment of perspective lines of barley on the main indicators of the quality of grain and efficiency during researches of 2011-2015 in the conditions of the southern forest-steppe of Western Siberia is given. The results of the conducted re-searches showed that for the formation of protein in grain optimum conditions were in 2012, starch - in 2011 and 2013, crude fat - in 2013 and 2015, the increased mass of 1000 grains - in 2011, 2014 and 2015, the productivity - in 2011 and 2015. The lowered filminess of grain was observed in 2011 and 2014. In control nursery in a complex of signs of quality of grain and efficiency filmy double-row lines Medikum 4860 are allocated, to Medikum+nutans 4777, Nutans 4847 and hull-less double-row line Nudum 4806. All listed lines differ in the increase in protein and productivity up to the standard, and also Medikum+nutans 4777 and Nudum 4806 had an increase according to the content of crude fat and on the mass of 1000 grains, Nutans 4847 was low-filmy. In collection nursery as gene sources of the raised protein con-tents and fineness of grain were allocated grades Hetman, Bezynchuksky 3, Volzhsky 65 and Vityaz. All the studied lines were analyzed by regression coefficient (bi), which determines the degree of response of genotypes to fluctuations in soil and climatic conditions (plasticity) and the degree of variability of comparable varieties, which is defined as the deviation from the regression line, (the level of stability of reactions σ2d). The varieties and lines were used in hybridization programs of the labora-tory of plant breeding of barley to create a new breeding material.