The research objective was the definition of im-pact of motor transport on the ecotoxicity of urbozem of Krasnoyarsk by phytotesting method. The researches were conducted in 2016 on the basis of laboratory of the Department of Ecology and Natural Sciences of Krasnoyarsk State Agricul-tural University. The influence on the intensity and structure of motor transportation stream of tempo-rary and seasonal factors was studied. The sam-ples of the soil were selected in 15-20 m from a road edge (Ave. Free and Vysotnaya St.) and as control - in 200 m from the road (residential district of Vetluzhanka). The soil was selected from the depth of 0-20 cm. Monocotyledonous plant -spring soft wheat (Triticum vulgare L.), grade Novosibirsk 29 and two-submultiple plant - garden cress (Lepidium sativum), the grade Spring miracle were used as test organisms. Urbozem ecotoxicity was established on the value of phytotoxicity index. The data obtained during the research showed that re-gardless of the season of the year the most part of motor transportation stream on Svobodny Ave. and Vysotnaya St. was made by cars - from 79.5 to 85.1 %. The total score of load of the main streets of Oktyabrsky district by motor transport was found as high. Negative impact of city soils on test cul-tures was established. The energy of germination and viability of seeds and the sizes of sprouts in comparison with control decreased considerably. By phytotesting method it was established that the urbozems of the area of researches were charac-terized by average degree of toxicity. The values of indexes of phytotoxicity on garden cress calculated on different test responses varied from 38.7 to 51.9 % for urbozem in Ave. Svobodny and from 32.1 to 46.5 % - in Vysotnaya St.; on wheat re-spectively from 41.0 to 58.2 % and from 35.0 to 56.3 %.