To analyze the dependence of the total content of antioxidants (TCA) in the Siberian grain varieties of oats on the genotype and cultivation conditions five samples of hulled and hull-less spring oats were investigated. The oats was grown for 2015 and 2016 in different geographic points: Krasno-turansky, Bay and Shira SSP. Weather conditions on options of experiment had both common fea- tures and distinctions by the amount of moisture and the modes of average daily temperatures by years, and also across territories of studying. In grain the extraction of tests for definition of TCA two eluent, i.e. hot bidistilled water or 70 % ethyl alcohol were used. The measurement of TCA in the samples was carried out using the device "Color Jauza-01-AA". As a sample comparison Gallic acid was used. The methods used for extraction with water and alcohol showed almost the same results. The maximum levels of TCA in grain were ob-served in oats grown in Beisky SSP, and the mini-mum in Krasnoturansk. The difference of TCA in the grain under the cultivation of oats in different years was almost missing. The maximum levels of TCA in grain were observed in the varieties of Ar-gument, and the lowest in the variety of Tubinsky. In the cultivation of oats in different years, but in one place TCA, all varieties were similar and changed in proportion to the ranking of varieties for SSA was not violated. While growing oats in differ-ent places of the TCA, all varieties have changed disproportionately, the ranking of varieties for TCA was significantly violated.