employee from 01.09.2025 to 01.09.2025
Kemerovo, Kemerovo, Russian Federation
Kemerovo, Russian Federation
Kemero
VAK Russia 4.1.1
VAK Russia 4.1.2
VAK Russia 4.1.3
VAK Russia 4.1.4
VAK Russia 4.1.5
VAK Russia 4.2.1
VAK Russia 4.2.2
VAK Russia 4.2.3
VAK Russia 4.2.4
VAK Russia 4.2.5
VAK Russia 4.3.5
UDC 631.8
UDC 631.559
The objective of the study is to conduct a comparative analysis of modern methods of biofortification of agricultural crops with selenium, and to evaluate their effectiveness, prospects, and limitations. The object of the study is agricultural crops fortified with selenium, as well as the agronomic (soil application, foliar feeding, hydroponics), biotechnological (genetic selection, use of microorganisms), and integrated biofortification methods applied to them. The subject of the study was quantitative indicators of the effectiveness of various biofortification methods, including the selenium assimilation coefficient (soil-to-plant transfer factor, STPTF), the proportion of organic forms of selenium in the yield, and the impact of the selected technologies on the agrobiological characteristics of plants. The methodology is based on the systematization and analysis of scientific publications, with an emphasis on quantitative indicators of effectiveness. Selenium assimilation coefficients (soil-to-plant transfer factor, STPTF) ranged from 0.2–0.7 for soil application to 0.7–0.9 in hydroponic systems. The share of organic selenium in the yield was 40–60 % for agrochemical methods and increased to 60–90 % when microbial inoculation and genetic modification were used. Foliar application increased selenium content in edible organs by 5–15 %, while the use of rhizosphere bacteria (e.g., Bacillus subtilis) increased selenomethionine concentration in wheat grain by 15 %. The greatest efficiency was achieved with integrated approaches combining foliar applications of nanosized forms of selenium (increasing Se content in tomato fruits by 75 %) with the use of selenium-accumulating varieties. Limitations of these methods include the dependence of agrochemical approaches on soil conditions, high cost, and regulatory barriers for biotechnological solutions. An optimal biofortification strategy requires a combination of methods taking into account the target crop, soil conditions, and economic factors, with potential lies in the development of precise dosing technologies and the creation of varieties with increased accumulation capacity.
biofortification, selenium, agronomic methods, biotechnological methods, absorption coefficient, organic selenium, integrated approaches
1. Tutelyan VA, Knyazhev VA, Khotimchenko SA, et al. Selen v organizme cheloveka: metabolizm, antioksidantnye svojstva, rol' v kancerogeneze. Moscow: Izd-vo RAMN; 2002. 219 p. (In Russ.).
2. Kriger PO, Mohova EV. Biologicheskaya rol` selena v pitanii sel`skoxozyajstvennyh zhivotnyh i pticy. In.: XIX Mezhdunarodnaya nauchno-prakticheskaya studencheskaya konferenciya «Himiya i zhizn`». Novosibirsk, 14 May 2020. 2020:58-62. (In Russ.).
3. Hossain A, Skalicky M, Brestic M, et al. Selenium biofortification: roles, mechanisms, responses and prospects. Molecules. 2021;26(4):881. DOI:https://doi.org/10.3390/molecules26040881.
4. Evstaf'eva EV, Golubkina NA, Boyarinceva YuA, et al. Obespechennost' selenom gorodskih zhitelej na territorii Krymskogo poluostrova. Gigiena i sanitariya. 2021;100(2):147-153. (In Russ.).
5. Buturi CV, Mauro R, Fogliano V, et al. Mineral biofortification of vegetables as a tool to improve human diet. Foods. 2021;10(2):223. DOI:https://doi.org/10.3390/foods10020223.
6. Gupta M, Gupta S. An overview of selenium uptake, metabolism, and toxicity in plants. Frontiers in plant science. 2017;7:2074. DOI:https://doi.org/10.3389/fpls.2016.02074.
7. Pan Z, Feng Ya, Wang M, et al. Geochemical characteristics of soil selenium and evaluation of selenium-rich land resources in Guiyang area. Frontiers in Geochemistry. 2023;1:1094023. DOI: 10.3389/ fgeoc.2023.1094023.
8. Zhao X, Lu Y, Dai L, et al. Selenium spatial distribution and bioavailability of soil-plant systems in China: A comprehensive review. Environmental Geochemistry and Health. 2024;46(9):341. DOI:https://doi.org/10.1007/s10653-024-02126-9.
9. Winkel L, Vriens B, Jones G, et al. Selenium cycling across soil-plant-atmosphere interfaces: a critical review. Nutrients. 2015;7(6):4199-4239. DOI:https://doi.org/10.3390/nu7064199.
10. Zhou Y, Nie K, Geng L, et al. Selenium’s role in plant secondary metabolism: regulation and mechanistic insights. Agronomy. 2024;15(1):54. DOI:https://doi.org/10.3390/agronomy15010054.
11. Jiang H., Lin W., Jiao H., et al. Uptake, transport, and metabolism of selenium and its protective effects against toxic metals in plants: A review. Metallomics. 2021;13(7). DOI:https://doi.org/10.1093/mtomcs/mfab040.
12. Trippe III RC, Pilon-Smits EAH. Selenium transport and metabolism in plants: Phytoremediation and biofortification implications. Journal of Hazardous Materials. 2021;404:124178. DOI:https://doi.org/10.1016/j.jhaz-mat.2020.124178.
13. Newman R, Waterland N, Moon Y, et al. Selenium biofortification of agricultural crops and effects on plant nutrients and bioactive compounds important for human health and disease prevention–a review. Plant Foods for Human Nutrition. 2019;74(4):449-460. DOI:https://doi.org/10.1007/s11130-019-00769-z.
14. Pobilat AE, Voloshin EI. Osobennosti soderzhaniya selena v sisteme pochva-rastenie (obzor). Bulletin of KSAU. 2020;11:98-105. (In Russ.).
15. Zhou X, Yang J, Kronzucker HJ, et al. Selenium biofortification and interaction with other elements in plants: a review. Frontiers in Plant Science. 2020;11:586421. DOI:https://doi.org/10.3389/fpls.2020.586421.
16. Golubkina NA, Zayachkovsky VA. Sravnitel'naya ocenka effektivnosti zashchity Raphanus sativus var. lobo ot krestocvetnoj gallicy (Contarinia nasturtii), ispol'zuya vnekornevoe vnesenie selenata natriya, ionnoj formy kremniya i ekstrakta chesnoka. Ovoshchi Rossii. 2024;(4):23-27. (In Russ.).
17. Bakaeva NP, Saltykova OL. Antistressovoe vozdejstvie organomineral'nyh udobrenij v agrotehnologii ozimoj pshenicy. Bulletin of KSAU. 2020;4:65-72. (In Russ.).
18. Voronin SP, Gumenyuk AP, Dubgorina EO, Tugarova AV, Evseeva NV, Yaroshenko TM., Es`kov ID, Denisov KE, Ryazancev NV. Organomineral`noe udobrenie i sposoby` ego primeneniya dlya sel`skohozyajstvennyh kul`tur. Patent RUS N2757604.2021. Byul. N 17. (In Russ.).
19. Liao Q, Xing Y, Li A-M, et al. Enhancing selenium biofortification: strategies for improving soil-to-plant transfer. Chemical and Biological Technologies in Agriculture. 2024;11(1):148. DOI: /10.1186/s40538-024-00672-z.
20. Danso OP, Asante-Badu B, Zhang Z, et al. Selenium biofortification: Strategies, progress and challenges. Agriculture. 2023;13(2):416. DOI:https://doi.org/10.3390/agriculture13020416.
21. Perfil'eva AI. Selensoderzhashchie nanostruktury: sintez, svojstva, agrohimicheskie aspekty primeneniya. Rossijskie nanotekhnologii. 2022;17(2):185-195. (In Russ.).
22. Puccinelli M, Malorgio F, Pezzarossa B. Selenium enrichment of horticultural crops. Molecules. 2017;22(6):933. DOI:https://doi.org/10.3390/molecules22060933.
23. Ponomareva SM, Semenova LI, Protunkevich IV. Issledovanie soderzhaniya selena v otechestven-nyh pishchevyh produktah. Innovacionnye tekhnologii proizvodstva i hraneniya material'nyh cennostej dlya gosudarstvennyh nuzhd. 2020;(13):183-193. (In Russ.).
24. Antoshkina MS, Golubkina NA, Bondareva LL. Vliyanie vnekornevogo obogashcheniya kapusty cvet-noj selenatom natriya na urozhajnost', pishchevuyu cennost' i antioksidantnyj status rastenij. Ovoshchi Rossii. 2020;(3):63-68. (In Russ.).
25. Shmykova NA, Suprunova TP, Pivovarov VF. Biotekhnologicheskie i molekulyarno-geneticheskie metody v selekcji ovoshchnyh kul'tur (k 95-letiyu VNIISSOK). Sel'skohozyajstvennaya biologiya. 2015;(5):561-570. (In Russ.).
26. Udincev SN, Zhilyakova TP. Sovremennye metody povysheniya pishchevoj cennosti sel'skohozyajst-vennoj produkcii. Vestnik Tomskogo gosudarstvennogo universiteta. Biologiya. 2012;2:81-91. (In Russ.).
27. Guo Q, Ye J, Zeng J, et al. Selenium species transforming along soil–plant continuum and their beneficial roles for horticultural crops. Horticulture research. 2023;10(2):uhac270. DOI:https://doi.org/10.1093/hr/uhac270.
28. Shangguan Y, Zhu J, Ye J, et al. Phytofortification: Enhanced Stress Resistance and Nutraceutical Enrichment in Horticultural Crops. Horticulture Research. 2025;12(12):236. DOI:https://doi.org/10.1093/hr/uhaf236.
29. Tangjaidee P, Swedlund P, Xiang J, et al. Selenium-enriched plant foods: Selenium accumulation, speciation, and health functionality. Frontiers in Nutrition. 2023;9:962312. DOI:https://doi.org/10.3389/fnut.2022.962312
30. Oancea AO, Gaspar A, Seciu A-M, et al. Development of a new technology for protective biofortification with selenium of Brassica crops. AgroLife Scientific Journal. 2015;4(2).
31. Schiavon M, Nardi S, Vecchia D, et al. Selenium biofortification in the 21st century: status and challenges for healthy human nutrition. Plant and soil. 2020;453(1):245-270. DOI:https://doi.org/10.1007/s11104-020-04635-9.
32. Deng G, Fan Zh, Wang Zh, et al. Dynamic role of selenium in soil–plant-microbe systems: Mechanisms, biofortification, and environmental remediation. Plant and Soil. 2025:1-21. DOI:https://doi.org/10.1007/s 11104-025-07661-7.
33. Pivovarov VF, Soldatenko IV, Pyshnaya ON, et al. Sovremennye tendencii razvitiya selekcji ovoshchnyh i bahchevyh kul'tur. Ovoshchi Rossii. 2022;(3):5-15. (In Russ.).
34. Huang J, Chen Y, Lang D, et al. Research progress on novel selenium biofortification technologies in agricultural soil-plant systems. Journal of Plant Nutrition and Fertilizers. 2025. DOI:https://doi.org/10.11674/zwyf. 2024544.
35. Li L, Liu QH, Yin CY. Selenium biofortification in plants and application potential of microorganisms in selenium biofortification. Chinese Journal of Plant Ecology. 2023;47(6):756. DOI:https://doi.org/10.17521/cjpe.2022.0163
36. Khakimova L, Vershinina ZR, Mslennikova DR, et al. Effects of Pseudomonas sp. OBA 2.4. 1 on Growth and Tolerance to Cadmium Stress in Pisum sativum L. BioTech. 2023;12(1):5. DOI: 10.3390/ biotech12010005.
37. Abadin ZU, Faisal M. Selenium resistant bacilli and pseudomonas as potential candidate for selenium and iron biofortification in maize plants. BioScientific Review. 2022;4(1):43-58. DOI:https://doi.org/10.32350/BSR. 0401.03.
38. Chen X, Zhang Z, Gu M, et al. Combined use of arbuscular mycorrhizal fungus and selenium fertilizer shapes microbial community structure and enhances organic selenium accumulation in rice grain. Science of the Total Environment. 2020;748:141166. DOI:https://doi.org/10.1016/j.scitotenv.2020.141166.
39. Kuzmina LY, Gilvanova E, Galimzyanova NF, et al. Characterization of the novel plant growth-stimulating strain Advenella kashmirensis IB-K1 and evaluation of its efficiency in saline soil. Microbiology. 2022;91(2):173-183. DOI:https://doi.org/10.1134/S0026261722020072.
40. Puccinelli M, Molorgio F, Incrocci L, et al. Effects of individual and simultaneous selenium and iodine biofortification of baby-leaf lettuce plants grown in two different hydroponic systems. Horticulturae. 2021;7(12):590. DOI:https://doi.org/10.3390/horticulturae7120590.
41. Golubkina NA, Seredin TA, Baranova EV, et al. Perspektivy polucheniya prorostkov semyan lukovyh kul'tur, obogashchennyh selenom. Ovoshchi Rossii. 2018;(6):50-54. (In Russ.). DOI: 10.18619/ 2072-9146-2018-6-50-54.
42. Ullah A, Yin X, Wang F, et al. Biosynthesis of selenium nanoparticles (via Bacillus subtilis BSN313), and their isolation, characterization, and bioactivities. Molecules. 2021;26(18):5559. DOI: 10.3390/ molecules26185559.
43. Li J, Gurijala H, Wu L, et al. Hyperaccumulator plants from China: a synthesis of the current state of knowledge. Environmental science & technology. 2018;52(21):11980-11994. DOI:https://doi.org/10.1021/acs.est. 8b01060.
44. Sindireva AV, Golubkina NA, Erdenedogot E. Kompleksnyj podhod k ocenke dejstviya selena v sisteme pochva-rastenie. Omsk: Publishing center KAN; 2022. 28 p. (In Russ.).
45. Zhang M, Xing G, Tang Sh, et al. Improving soil selenium availability as a strategy to promote selenium uptake by high-Se rice cultivar. Environmental and Experimental Botany. 2019;163:45-54. DOI:https://doi.org/10.1016/j.envexpbot.2019.04.008
46. Tsioubri M, Gasparatos D, Economou-Eliopoulos M. Selenium uptake by Lettuce (Lactuca sativa L.) and Berseem (Trifolium alexandrinum L.) as affected by the application of sodium selenate, soil acidity and organic matter content. Plants. 2020;9(5):605. DOI:https://doi.org/10.3390/plants9050605.
47. Shiriaev A, Pezzarossa B, Rosellini I, et al. Efficacy and comparison of different strategies for sele-nium biofortification of tomatoes. Horticulturae. 2022;8(9):800. DOI:https://doi.org/10.3390/horticulturae8090800.
48. Sabatino L, Ntatsi G, Lapichino G, et al. Effect of selenium enrichment and type of application on yield, functional quality and mineral composition of curly endive grown in a hydroponic system. Agronomy. 2019;9(4):207. DOI:https://doi.org/10.3390/agronomy9040207.
49. Puccinelli M, Malorgio F, Maggini R, et al. Biofortification of Ocimum basilicum L. plants with sele-nium. In: III International Symposium on Horticulture in Europe-SHE2016. 2016:663-670. DOI:https://doi.org/10.17660/ActaHortic.2019.1242.98.
50. Amagova ZA, Golubkina NA. Effektivnost' ispol'zovaniya selenata natriya pri vyrashchivanii tomata v usloviyah oksidantnogo stressa. Ovoshchi Rossii. 2018;(1):79-81. (In Russ.).
