student
Federal'nyy nauchnyy centr bioraznoobraziya nazemnoy bioty Vostochnoy Azii DVO RAN
Vladivostok, Vladivostok, Russian Federation
Vladivostok, Vladivostok, Russian Federation
Vladivostok, Vladivostok, Russian Federation
VAK Russia 1.5.20
VAK Russia 4.1.1
VAK Russia 4.1.2
VAK Russia 4.1.3
VAK Russia 4.1.4
VAK Russia 4.1.5
VAK Russia 4.2.1
VAK Russia 4.2.2
VAK Russia 4.2.3
VAK Russia 4.2.4
VAK Russia 4.2.5
VAK Russia 4.3.3
VAK Russia 4.3.5
UDC 57
UDC 574/577
CSCSTI 34.15
Russian Classification of Professions by Education 06.04.01
Russian Library and Bibliographic Classification 280
Russian Trade and Bibliographic Classification 6412
The aim of the study is to investigate the qualitative and quantitative composition of stilbenes in young spring seedlings of Japanese knotweed (Reynoutria japonica). Research was conducted at the Federal Scientific Center for Biodiversity of Terrestrial Biota of East Asia, Far Eastern Branch of the Russian Academy of Sciences (Russia). The objects of the study were spring seedlings of R. japonica, collected in the spring of 2025 from the arboretum of the Gornotaezhnaya Station, a branch of the Federal Scientific Center for Biodiversity, Far Eastern Branch of the Russian Academy of Sciences (Primorsky Region, Russia). The material was collected during the active growth phase of the seedlings, which minimized the influence of ontogenetic heterogeneity on the chemical composition of the samples. A total of three seedlings were collected from each of the eight maternal plants; the combined samples from each plant were considered independent biological replicates. High-performance liquid chromatography with ultraviolet and mass spectrometric detection (HPLC-UV-MS/MS) was used as the primary research method. For HPLC-UV-MS/MS analysis, R. japonica shoots were dried at 60 °C for 48 h and ground in an IKA A 10 basic laboratory mill. Stilbenes extraction conditions were optimized, including solvent selection (methanol, ethanol, water), temperature (20, 40, 60 °C), and process duration (2, 4, 6 h). Young seedlings were characterized by exceptionally high stilbenes content. The main identified compounds were resveratrol, trans-polydatin, and trans-resveratrol. The highest extraction efficiency was achieved using 70 % methanol and 70 % ethanol (up to 78 and 86.6 mg/g dry weight, respectively) for 2 h, and the optimal parameters were a temperature of 60 °C and an extraction time of 6 h. Under optimal conditions, the total stilbenes content from R. japonica seedlings reached 95 mg/g dry weight, which is comparable to values previously recorded for this and other plant species. Spring sprouts of Japanese knotweed (R. japonica) are one of the richest known natural sources of stilbenes.
stilbenes, HPLC-MS/MS, Japanese knotweed, knotweed, seasonal variations
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