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The aim of the study is to evaluate the phosphate-mobilizing activity of microflora from the main zonal soil types in the Ryazan Region. Soil samples from the main zonal soil types in the Ryazan Region were analyzed: sod-podzolic soil (Kasimovsky District, Ryazan Region), gray forest soil (Ryazan District, Ryazan Region), and leached chernozem (Aleksandro-Nevsky District, Ryazan Region). Phosphate-solubilizing mic¬roorganism strains were isolated using various nutrient media: cultures were inoculated on both a selective nutrient medium (liquid and solid) and a general-purpose medium, followed by subculture on a selective medium. Enterobacter ludwigii, a well-known phosphate mobilizer used in agricultural practice, was used as a positive control. The key criterion for assessing the activity of the isolated microorganisms was the concentration of phosphorus mobilized by them, which was quantified spectrophotometrically. Microorganisms isolated from sod-podzolic soil and leached chernozem in the Ryazan Region showed maximum phosphate-mobilizing activity, exceeding that of even the reference strain Enterobacter ludwigii. Thus, in the experimental variant with Enterobacter ludwigii, the soluble phosphorus concentration was 287 mg/l, while in the variants with leached chernozem and sod-podzolic soil, it was 307 and 361 mg/l, respectively. Phosphorus concentrations released by bacteria in leached chernozem exceeded those of the reference phosphate mobilizer Enterobacter ludwigii by 7 % over 20 days of exposure, and for strains from sod-podzolic soil, the diffe¬rence was more than 25 %. The obtained data demonstrate the potential of using native microorganisms adapted to local soil conditions for the development of biopreparations aimed at increasing phosphorus availability to plants.
microbiological phosphate mobilization, leached chernozem, gray forest soil, sod-podzolic soil, bacilli, enterobacteria
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